Journal: Frontiers in Endocrinology
Article Title: Quantitative Ubiquitinomics Revealed Abnormal Ubiquitinated ATP7A Involved in Down-Regulation of ACTH in Silent Corticotroph Adenomas
doi: 10.3389/fendo.2022.863017
Figure Lengend Snippet: Function of ATP7A in AtT20 cells. (A) The intensity of ubiquitinated peptides of ATP7A in SCAs and FCAs. (B) Up-regulation of the ACTH level in AtT20 cells with the knockdown of ATP7A by ATP7A siRNA. (C) Up-regulation of the ACTH level in AtT20 cells treated with 20uM omeprazole. All assays were performed in triplicate. (D) The top 20 potential E3 ligases of ATP7A. *Compared to control, p < 0.05.
Article Snippet: AtT20 cells treated with omeprazole (HY-B0113A, MedChemExpress) and the ACTH levels were assessed by an enzyme-linked immunosorbent assay (ELISA) (SBJ-M0483, SBJbio) according to the manufacture’s protocol.
Techniques: Knockdown, Control